研究成果： |
承担的项目： 国家自然科学基金：嗜热酰基氨肽酶非特异性催化机制的研究及改造 吉林省自然科学基金：高温糖基化生物反应器的构建 国家重点研发计划项目子课题:基于酶和工程菌改造技术的分布式DNA活字存储阵列研究
2019年以来课题组发表文章： [1] Zihao Huang, Zhenyu Gu, Xiemuxinuer Abuduwupuer, Deyuan Qin, Yuchen Liu, Zheng Guo*, Renjun Gao*, Engineering non-conservative substrate recognition sites of extradiol dioxygenase: Computation guided design to diversify and accelerate degradation of aromatic compounds, International Journal of Biological Macromolecules, 2024, 264, 130739 [2] Xiuhui Tang, Lijuan Zhang, Mingwang Huang, Fang Wang, Guiqiu Xie, Rui Huo, Renjun Gao*, Selective enhanced cytotoxicity of amino acid deprivation for cancer therapy using thermozyme functionalized nanocatalyst, Journal of Nanobiotechnology, 2024, 22, 53. [3] Yajing Li, Qi Chen, Siyao Liu, Lin Deng, Shichao Li, and Renjun Gao*, Efficient one-pot synthesis of uridine diphosphate galactose employing a tri-enzyme system, Journal of Agricultural and Food Chemistry. 2024, 72, 3644−3653 [4] Yiwen Xu, Xiaoran Xuan, Renjun Gao, Guiqiu Xie*, Increased expression levels of thermophilic serine protease TTHA0724 through signal peptide screening in bacillus subtilis and applications of the enzyme, International Journal of Molecular Sciences, 2023, 24, 15950. [5] Ye Zhou, Jannik Nedergaard Pedersen, Jacob Nedergaard Pedersen, Nykola C. Jones, Søren Vrønning Hoffmann, Steen Vang Petersen, Jan Skov Pedersen, Adam Perriman, Renjun Gao*, and Zheng Guo*，Superanionic solvent-free liquid enzymes exhibit enhanced structures and activities, Advanced. Science. 2022, 9, 2202359. [6] Abidan Ainiwaer, Ao Li, Xingwang Zhao, Yujiao Xu, Siping Han*, and Renjun Gao*, Site-specific covalent immobilization of Methylobacterium extorquens non-blue laccse melac13220 on Fe3O4 nanoparticles by aldehyde Tag, Catalysts. 2022, 12, 1379. [7] Abidan Ainiwaer, Yue Liang, Xiao Ye and Renjun Gao*, Characterization of a Novel Fe2+ Activated Non-Blue Laccase from Methylobacterium extorquens, International Journal of Molecular Sciences. 2022, 23, 9804. [8] Yajing Li, Xue Yang and Renjun Gao*, Thermophilic inorganic pyrophosphatase ton1914 from Thermococcus onnurineus NA1 removes the inhibitory effect of pyrophosphate, International Journal of Molecular Sciences. 2022, 23, 12735. [9] Fang Wang, Yuchen Liu, Chang Du and Renjun Gao*, Current strategies for real-time enzyme activation, Biomolecules, 2022, 12, 599. [10] Jiabao Lyu, Jian Zhang, Jingxuan Zhu, Siao Chen, Tao Han, Yan Zhang, Renjun Gao, Guiqiu Xie*, Zheng Guo*, Molecular dynamics simulation guided distal mutation of Thermotoga naphthophila β-glucosidase for significantly enhanced synthesis of galactooligosaccharides and expanded product scope, International Journal of Biological Macromolecules, 2022, 210, 21-32. [11] Xiaowen Zhang, Zihao Huang, Dan Wang, Yan Zhang, Bekir Engin Eser, Zhenyu Gu, Rongrong Dai, Renjun Gao*, Zheng Guo*, A new thermophilic extradiol dioxygenase promises biodegradation of catecholic pollutants, Journal of Hazardous Materials, 422 (2022), 126860. [12] Li Zong, Yan Zhang, Zhengkang Shao, Yingwu Wang, Zheng Guo, Renjun Gao*, Bekir Engin Eser*, Optimization and engineering of a self-sufficient cyp102 enzyme from Bacillus amyloliquefaciens towards synthesis of in-chain hydroxy fatty acids, Catalysts, 2021, 11(6), 665. [13] Li Zong, Renjun Gao, Zheng Guo, hengkang Shao, Yingwu Wang*, Bekir EnginEser*, Characterization and modification of two self-sufficient CYP102 family enzymes from Bacillus amyloliquefaciens DSM 7 with distinct regioselectivity towards fatty acid hydroxylation, Biochemical Engineering Journal, 2021, 166, 107871. [14] Jiabao Lyu, Renjun Gao, and Zheng Guo*, Galactosyldiacylglycerols: From a Photosynthesis-Associated Apparatus to Structure-Defined In Vitro Assembling, Journal of Agricultural and Food Chemistry. 2021, 69, 32, 8910-8928. [15] Fang Wang, Rong Li, Hui Jian, Zihao Huang, Yingwu Wang*, Zheng Guo*, and Renjun Gao*, Design and construction of an effective expression system with aldehyde tag for site-specific enzyme immobilization, Catalysts, 2020, 10(4), 410. [16] Dongni Liu, Lin Deng, Dan Wang, Wei Li, Renjun Gao*，“Bridge regions” regulate catalysis and protein stability of acylpeptide hydrolase, Biochemical Engineering Journal, 2019, 145, 42-52. [17] Wei Li, Dongni Liu, Xu Geng, Zhengqiang Li* and Renjun Gao*, Real-time regulation of catalysis by remote-controlled enzyme-conjugated gold nanorod composites for aldol reaction-based applications, Catalysis Science & Technology, 2019, 9, 2221-2230. [18] Ye Zhou, Bianca Pérez, Weiwei Hao, Jiabao Lv, Renjun Gao*, Zheng Guo*, The additive mutational effects from surface charge engineering: A compromise between enzyme activity, thermostability and ionic liquid tolerance, Biochemical Engineering Journal, 2019, 148,195-204. [19] Ye Zhou, Nykola C. Jones, Jannik Nedergaard Pedersen, Bianca Pérez, S. V. Hoffmann, Steen Vang Petersen, Jan Skov Pedersen, Adam Perriman, Peter Kristensen, Renjun Gao*, and Zheng Guo*, Insight into the Structure and Activity of Surface-Engineered Lipase Biofluids, ChemBioChem, 2019, 20, 1266-1272. [20] Jiabao Lyu, Zidan Li, Juncheng Men, Ruyun Jiang, Guilin Tang, Ye Zhou, Renjun Gao*, Covalent immobilization of Bacillus subtilis lipase A on Fe3O4 nanoparticles by aldehyde tag: An ideal immobilization with minimal chemical modification, Process Biochemistry, 2019, 81, 63-69. [21] Dongni Liu, Wei Li, Xiaoyu Jiang, Shuju Bai, Jiyang Liu, Xutong Liu, Yuhua Shi, Ziyu Kuai, Wei Kong, Renjun Gao* and Yaming Shan*, Using near-infrared enhanced thermozyme and scFv dual-conjugated Au nanorods for detection and targeted photothermal treatment of Alzheimer’s disease, Theranostics, 2019, 9(8), 2268-2281.
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